The present manuscript describes simple, sensitive, rapid, accurate, precise and economical derivative spectroscopic method for the simultaneous determination of nifedipine and lidocaine in cream. Derivative spectroscopy offers a useful approach for the analysis of drugs in cream. In this study a second derivative spectroscopic method was used for simultaneous determination of nifedipine and lidocaine using the zero-crossing technique. The measurements were carried out at wavelengths of 259.2 nm and 245.2 nm for nifedipine and lidocaine respectively. The method was found to be linear (r2 = 0.997) in the range of 4-20 μg/ml for nifedipine at 259.2 nm. The linear correlation was obtained (r2 = 0.999) in the range of 20-100 μg/ml for lidocaine at 245.2 nm. The limit of detection was found and 0.77 μg/ml for nifedipine and lidocaine respectively. The limit of quantification was found to be 1.19 and 2.34 μg/ml respectively. The method was successfully applied for simultaneous determination of nifedipine and lidocaine in cream.
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